DNA Structure and Gene Expression Flashcards
select the findings of Erwin Chargaff regarding DNA composition.
Discovery of the structure of DNA (article)
One other key piece of information related to the structure of DNA came from Austrian biochemist Erwin Chargaff. Chargaff analyzed the DNA of different species, determining its composition of A, T, C, and G bases. He made several key observations: A, T, C, and G were not found in equal quantities (as some models at the time would have predicted).
The following table is a representative sample of Erwin Chargaff’s 1952 data, listing the base composition of DNA from various organisms and support both of Chargaff’s rules. An organism such as φX174 with significant variation from A/T and G/C equal to one, is indicative of single stranded DNA.
14.1A: Discovery of DNA
Erwin Chargaff met Francis Crick and James D. Watson at Cambridge in 1952, and, despite not getting along with them personally, he explained his findings to them. Chargaff’s Rule showed that in natural DNA, the number of guanine units equals the number of cytosine units and the number of adenine units equals the number of thymine units.
Chargaff's Rules: the Work of Erwin Chargaff
The paper describing Chargaff’s analytical method is reprinted here as a Journal of Biological Chemistry (JBC) Classic. His procedureconsisted of three steps. The first was the separation of the DNA mixture intoindividual components by paper chromatography. Next, the separated compoundswere converted into mercury salts.
Discovery of the Structure of DNA
The experiments done by Erwin Chargaff, Rosalind Franklin, James Watson and Francis Crick led to the discovery of the structure of DNA and the beginning of modern genetics, which lead to a greater understanding of diseases in society like cancer and how to prevent them from invading communities and taking human lives. Background.
Chargaff's BasePairing Rules
Summary. Chargaff’s rule 1 is that the number of guanine units approximately equals the number of cytosine units and the number of adenine units approximately equals the number of thymine units. Chargaff’s rule 2 is that the composition of DNA varied from one species to another. The base paring rules state that A always pairs with T and G.
Discovery of DNA Structure and Function: Watson and Crick
Erwin Chargaff was one of a handful of scientists who expanded on Levene’s work by uncovering additional details of the structure of DNA, thus further paving the way for Watson and Crick.
Biochemical information obtained by Erwin Chargaff regarding the 1:1 stoichiometry of G:C and A:T in DNA extracted from a wide range of organisms also facilitated Watson and Crick’s elucidation of the double-helix structure (Elson and Chargaff 1954). Crick, Watson, and Wilkins shared the 1962 Nobel Prize in Physiology and Medicine for their.
Erwin Chargaff biochemist
genetics research. In heredity: Structure and composition of DNA. …it was found by biochemist Erwin Chargaff that the amount of A is always equal to T, and the amount of G is always equal to C. Read More. Other articles where Erwin Chargaff is discussed: heredity: Structure and composition of DNA: …it was found by biochemist Erwin Chargaff.