Parts of a Microscope Flashcards
What two structures on the microscope will you use to focus on your specimen? The coarse and fine adjustment knob Why should you never use the coarse adjustment knob on high power? Because it will crack your slide What will happen if you use direct sunlight to observe your specimen? It may damage your eyes Our microscopes have three objectives.
3.1: Introduction to the Microscope
Objective lenses: Initial magnification of your specimen occurs here. Most brightfield light microscopes have 3 objective lenses seated into the resolving nose piece base. 4. Coarse focusing knob: larger of the two knobs, the coarse adjustment knob moves the stage up or down to bring the specimen into focus. It is very sensitive, even small.
On the side of the microscope are two knobs, one on top of the other. The larger of the two knobs is the coarse focus adjustment knob. Turn the knob so that the stage goes down as far as it can. Clean all lenses with lens paper. Never use paper towels or kimwipes or shirt! Obtain a letter “e” slide from your instructor.
Look through the microscope and use the course focus knob to focus on the sample. Make sure the slide is in very sharp focus before moving on. Check with your instructor if you are uncertain. To focus on the specimen using higher magnification than with the 4X objective, use the next steps:.
Microscopy: Intro to microscopes & how they work (article
Magnification is a measure of how much larger a microscope (or set of lenses within a microscope) causes an object to appear. For instance, the light microscopes typically used in high schools and colleges magnify up to about 400 times actual size. So, something that was 1 mm wide in real life would be 400 mm wide in the microscope image.
A microscope is an instrument that magnifies an object so that it may be seen by the observer. Because cells are usually too small to see with the naked eye, a microscope is an essential tool in the field of biology. In addition to magnification, microscopes also provide resolution, which is the ability to distinguish two nearby objects as.
1.5: Setting Up a Microscope and Slide Properly
2. Pick up microscope by carrying arm, position it so it is accessible to your seat, with open side of the stage facing you. 3. Rotate the objectives so that the lowest power objective (smallest in size) clicks into place. 4. Look at the slide with your naked eye and find the location of the specimen. 5.
Microscopes and Cells
Use two hands to carry the microscope. Place one hand under it to support its weight, and hold onto the handle on the back of the microscope arm. If your microscope does not have a handle, hold tightly to the arm itself. Cleaning the oculars and objective lenses. If your microscope lenses are dirty, then the view of your specimen will be obscured.
Clean the lenses with lens paper (if needed). The lenses on the microscope scratch easily. If you need to clean them, use ONLY lens paper (KIM Wipes are not lens paper). Before you use the microscope, locate the parts of the microscope and label Figure 1.2.1 1.2.
Looking at the Structure of Cells in the Microscope
The phase-contrast microscope and, in a more complex way, the differential-interference-contrast microscope, exploit the interference effects produced when these two sets of waves recombine, thereby creating an image of the cell’s structure . Both types of light microscopy are widely used to visualize living cells.